Methods for the large-scale synthesis of psoralen furan-side monoadducts and diadducts.

Spielmann, H P; Sastry, S S; Hearst, J E

Methods for the large-scale synthesis of psoralen furan-side monoadducts and diadducts.

Authors

Spielmann, H P

Sastry, S S

Hearst, J E

Publisher

National Academy of Sciences

Description

We report methods for the preparation of multimicromole quantities of extremely pure uniquely photo-adducted psoralen-DNA furan-side monoadducts and diadducts (cross-links). The methods use high-intensity krypton and argon ion lasers in the photoreactions and HPLC methods to purify the required oligonucleotides containing the photoadducts. With these methods we have synthesized 2-3 mumol of 8-mer psoralen furan-side monoadduct and diadduct. These methods allow one to generate large amounts of psoralenated DNA oligonucleotides and facilitate their study by NMR and x-ray crystallography. IMAGES: