Methods for the large-scale synthesis of psoralen furan-side monoadducts and diadducts.
| dc.creator | Spielmann, H P | |
| dc.creator | Sastry, S S | |
| dc.creator | Hearst, J E | |
| dc.date | 1992-05-15 | |
| dc.date.accessioned | 2026-08-03T01:33:42Z | |
| dc.description | We report methods for the preparation of multimicromole quantities of extremely pure uniquely photo-adducted psoralen-DNA furan-side monoadducts and diadducts (cross-links). The methods use high-intensity krypton and argon ion lasers in the photoreactions and HPLC methods to purify the required oligonucleotides containing the photoadducts. With these methods we have synthesized 2-3 mumol of 8-mer psoralen furan-side monoadduct and diadduct. These methods allow one to generate large amounts of psoralenated DNA oligonucleotides and facilitate their study by NMR and x-ray crystallography. IMAGES: | |
| dc.identifier | https://pmc.ncbi.nlm.nih.gov/articles/PMC49113/ | |
| dc.identifier | https://pubmed.ncbi.nlm.nih.gov/1584785/ | |
| dc.identifier | https://doi.org/10.1073/pnas.89.10.4514 | |
| dc.identifier.uri | https://repo.dare.co.zw/handle/123456789/155871 | |
| dc.language | en | |
| dc.publisher | National Academy of Sciences | |
| dc.source | Proc Natl Acad Sci U S A | |
| dc.subject | Research Article | |
| dc.title | Methods for the large-scale synthesis of psoralen furan-side monoadducts and diadducts. | |
| dc.type | Text |