Estimation of the DNA sequence discriminatory ability of hairpin-linked lexitropsins

Walker, Wynn L.; Landaw, Elliot M.; Dickerson, Richard E.; Goodsell, David S.

Estimation of the DNA sequence discriminatory ability of hairpin-linked lexitropsins

Authors

Walker, Wynn L.

Landaw, Elliot M.

Dickerson, Richard E.

Goodsell, David S.

Publisher

National Academy of Sciences

Description

Three- and four-ring polyamides containing N-methylimidazole and N-methylpyrrole, and their hairpin-linked derivatives, bind side-by-side in the minor groove of DNA in a sequence-specific manner. The sequences recognized by side-by-side molecules are dependent on the pairings of the polyamide rings to the bases. In this study we report a mathematical model for estimating the free energies of binding for γ-aminobutyric acid-linked polyamides to 5- and 6-bp DNA sequences. The model parameters are calibrated by a least-squares fit to 35 experimental binding constants. The model performs well in cross-validation experiments and the parameters are consistent with previously proposed empirical rules of polyamide–DNA binding. We apply the model to the design of targeted polyamides, evaluating the ability of the proposed polyamides to bind to a DNA sequence of interest while minimizing binding to the remaining DNA sequences.