Imaging Glycosylation In Vivo by Metabolic Labeling and Magnetic Resonance Imaging.

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dc.creatorNeves, André A
dc.creatorWainman, Yéléna A
dc.creatorWright, Alan
dc.creatorKettunen, Mikko I
dc.creatorRodrigues, Tiago B
dc.creatorMcGuire, Sarah
dc.creatorHu, De-En
dc.creatorBulat, Flaviu
dc.creatorGeninatti Crich, Simonetta
dc.creatorStöckmann, Henning
dc.creatorLeeper, Finian J
dc.creatorBrindle, Kevin M
dc.date2015-11-16T15:52:10Z
dc.date2015-11-16T15:52:10Z
dc.date2016-01-22
dc.date.accessioned2026-08-03T04:14:29Z
dc.descriptionGlycosylation is a ubiquitous post-translational modification, present in over 50% of the proteins in the human genome, with important roles in cell-cell communication and migration. Interest in glycome profiling has increased with the realization that glycans can be used as biomarkers of many diseases, including cancer. We report here the first tomographic imaging of glycosylated tissues in live mice by using metabolic labeling and a gadolinium-based bioorthogonal MRI probe. Significant N-azidoacetylgalactosamine dependent T1  contrast was observed in vivo two hours after probe administration. Tumor, kidney, and liver showed significant contrast, and several other tissues, including the pancreas, spleen, heart, and intestines, showed a very high contrast (>10-fold). This approach has the potential to enable the rapid and non-invasive magnetic resonance imaging of glycosylated tissues in vivo in preclinical models of disease.
dc.descriptionThe work was supported by a Cancer Research UK Programme grant (17242) to KMB and the CRUK-EPSRC Imaging Centre in Cambridge and Manchester (16465). Y.A.W. and F.B. were in receipt of Cancer Research UK Ph.D. studentships.
dc.descriptionThis is the final version of the article. It first appeared from Wiley via http://dx.doi.org/10.1002/anie.201509858
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dc.identifierNeves et al. Angewandte Chemie International Edition (2015) Vol. 55, pp. 1286-1290. doi:10.1002/anie.201509858
dc.identifier1433-7851
dc.identifierhttps://www.repository.cam.ac.uk/handle/1810/252624
dc.identifier1521-3773
dc.identifier.urihttps://repo.dare.co.zw/handle/123456789/186782
dc.languageEnglish
dc.languageeng
dc.publisherWiley
dc.publisherhttps://doi.org/10.1002/anie.201509858
dc.rightsAttribution 2.0 UK: England & Wales
dc.rightshttps://creativecommons.org/licenses/by/2.0/uk/
dc.subjectbioorthogonal chemistry
dc.subjectcancer
dc.subjectgadolinium
dc.subjectglycans
dc.subjectmagnetic resonance imaging
dc.subjectAnimals
dc.subjectCarbohydrates
dc.subjectGadolinium
dc.subjectGlycosylation
dc.subjectMagnetic Resonance Imaging
dc.subjectMice
dc.subjectMolecular Probes
dc.subjectTissue Distribution
dc.titleImaging Glycosylation In Vivo by Metabolic Labeling and Magnetic Resonance Imaging.
dc.typeArticle

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