Cloning and expression of the tumor-associated antigen L6.

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dc.creatorMarken, J S
dc.creatorSchieven, G L
dc.creatorHellström, I
dc.creatorHellström, K E
dc.creatorAruffo, A
dc.date1992-04-15
dc.date.accessioned2026-08-03T01:32:39Z
dc.descriptionThe L6 cell surface antigen, which is highly expressed on lung, breast, colon, and ovarian carcinomas, has attracted attention as a therapeutic target for murine monoclonal antibodies and their humanized counterparts. Its molecular nature has, however, remained elusive. Here we describe the expression cloning of a cDNA encoding the L6 antigen. COS cells transfected with this cDNA direct the expression of an approximately 24-kDa surface protein that reacts with the two anti-L6 monoclonal antibodies available. The predicted L6 peptide sequence is 202 amino acids long and contains three predicted NH2-terminal hydrophobic transmembrane regions, which are followed by a hydrophilic region containing two potential N-linked glycosylation sites and a COOH-terminal hydrophobic transmembrane region. The L6 antigen is related to a number of cell surface proteins with similar predicted membrane topology that have been implicated in cell growth. Two other members of this family of proteins, CD63 (ME491) and CO-029, are also highly expressed on tumor cells. The present findings should make it possible to further study the role of the L6-defined antigen in normal and neoplastic cells and to construct animal models for development of improved agents for active and passive cancer immunotherapy. IMAGES:
dc.identifierhttps://pmc.ncbi.nlm.nih.gov/articles/PMC48896/
dc.identifierhttps://pubmed.ncbi.nlm.nih.gov/1565644/
dc.identifierhttps://doi.org/10.1073/pnas.89.8.3503
dc.identifier.urihttps://repo.dare.co.zw/handle/123456789/155566
dc.languageen
dc.publisherNational Academy of Sciences
dc.sourceProc Natl Acad Sci U S A
dc.subjectResearch Article
dc.titleCloning and expression of the tumor-associated antigen L6.
dc.typeText

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