A putative ATP-dependent RNA helicase involved in Saccharomyces cerevisiae ribosome assembly.
| dc.creator | Ripmaster, T L | |
| dc.creator | Vaughn, G P | |
| dc.creator | Woolford, J L | |
| dc.date | 1992-12-01 | |
| dc.date.accessioned | 2026-08-03T01:49:08Z | |
| dc.description | We have isolated a cold-sensitive mutant of Saccharomyces cerevisiae in which there is a deficit of 60S ribosomal subunits. Cold sensitivity and the assembly defect are recessive and cosegregate, defining a single essential gene that we designated DRS1 (deficiency of ribosomal subunits). The wild-type DRS1 gene was cloned by complementation of the cold-sensitive phenotype of drs1. Sequence analysis reveals a high degree of similarity to a family of proteins that are thought to function as ATP-dependent RNA helicases. Pulse-chase analysis of ribosomal RNA synthesis and processing indicates that the drs1 mutant accumulates the 27S precursor of the mature 25S rRNA. These results suggest that, as in pre-mRNA splicing, RNA helicase activities are involved in ribosomal RNA processing. IMAGES: | |
| dc.identifier | https://pmc.ncbi.nlm.nih.gov/articles/PMC50503/ | |
| dc.identifier | https://pubmed.ncbi.nlm.nih.gov/1454790/ | |
| dc.identifier | https://doi.org/10.1073/pnas.89.23.11131 | |
| dc.identifier.uri | https://repo.dare.co.zw/handle/123456789/159998 | |
| dc.language | en | |
| dc.publisher | National Academy of Sciences | |
| dc.source | Proc Natl Acad Sci U S A | |
| dc.subject | Research Article | |
| dc.title | A putative ATP-dependent RNA helicase involved in Saccharomyces cerevisiae ribosome assembly. | |
| dc.type | Text |