Murine monoclonal anti-idiotope antibody breaks unresponsiveness and induces a specific antibody response to human melanoma-associated proteoglycan antigen in cynomolgus monkeys.
| dc.creator | Chattopadhyay, P | |
| dc.creator | Starkey, J | |
| dc.creator | Morrow, W J | |
| dc.creator | Raychaudhuri, S | |
| dc.date | 1992-04-01 | |
| dc.date.accessioned | 2026-08-03T01:30:36Z | |
| dc.description | The mouse monoclonal antibody MEM136 (mAb1) is directed against an epitope on human melanoma-associated proteoglycan antigen (MPG). This epitope is also present on various normal human and subhuman tissues. A monoclonal murine anti-idiotope (anti-Id) antibody (mAb2), designated I-Mel-2, was generated against MEM136 and used as a surrogate antigen for the MPG molecule. I-Mel-2 was tested in cynomolgus monkeys (Macaca fascicularis) for its ability to induce anti-MPG humoral responses. All monkeys immunized with Ab2 developed specific anti-anti-idiotype (Ab3) responses that were capable of inhibiting binding of Ab2 to Ab1. Furthermore, I-Mel-2 immune monkey serum contained anti-MPG antibodies (Ab1') that bound to MPG-positive but not to MPG-negative melanoma cell lines. Monkeys immunized with Colo38 melanoma cells (membrane-bound MPG antigen) did not contain anti-MPG antibodies that inhibited the binding of two distinct anti-MPG mAb 125I-labeled MEM136 or 125I-labeled 225.28 to Colo38 cells. The induction of anti-MPG responses in monkeys did not cause any apparent side effects in animals, despite the fact that the MPG antigen is expressed by many normal tissues. The affinity-purified, I-Mel-2 idiotype-specific, Ab3 immunoprecipitated MPG antigen from melanoma cells. Furthermore, the I-Mel-2-induced Ab3 inhibited melanoma cell invasion in an in vitro assay, implying that these antibodies have biological significance. IMAGES: | |
| dc.identifier | https://pmc.ncbi.nlm.nih.gov/articles/PMC48726/ | |
| dc.identifier | https://pubmed.ncbi.nlm.nih.gov/1557374/ | |
| dc.identifier | https://doi.org/10.1073/pnas.89.7.2684 | |
| dc.identifier.uri | https://repo.dare.co.zw/handle/123456789/155012 | |
| dc.language | en | |
| dc.publisher | National Academy of Sciences | |
| dc.source | Proc Natl Acad Sci U S A | |
| dc.subject | Research Article | |
| dc.title | Murine monoclonal anti-idiotope antibody breaks unresponsiveness and induces a specific antibody response to human melanoma-associated proteoglycan antigen in cynomolgus monkeys. | |
| dc.type | Text |