Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies.
| dc.creator | Nakamura, T | |
| dc.creator | Kubagawa, H | |
| dc.creator | Cooper, M D | |
| dc.date | 1992-09-15 | |
| dc.date.accessioned | 2026-08-03T01:42:44Z | |
| dc.description | Two covalently linked transmembrane molecules, encoded in mice by the mb-1 and B29 genes, have been defined as integral components of the antibody receptor units expressed on B cells. We have produced monoclonal antibodies against an exposed extracellular epitope on the putative human equivalent of the mouse B29 product. These antibodies, CB3-1 and -2, were used to show that cytoplasmic expression of this molecule begins in human pro-B cells (terminal deoxynucleotidyltransferase-positive, mu chain-negative), whereas surface expression coincides strictly with surface immunoglobulin expression of all isotypes. Immunochemical analysis of the human immunoglobulin-associated molecules revealed greater molecular heterogeneity than has been noted for the murine analogues. This molecular heterogeneity of immunoglobulin-associated molecules varied as a function of differentiation stage and the immunoglobulin isotypes expressed by B-lineage cells. Our data support the hypothesis that biochemical heterogeneity of the surface immunoglobulin-associated molecules may contribute to the variability in biological effects of antigen receptor crosslinkage on B cells of different maturational stages. Because the CB3 antibodies are capable of down-modulating the antigen receptors on all B cells, they may prove therapeutically useful as universal B-cell suppressants. IMAGES: | |
| dc.identifier | https://pmc.ncbi.nlm.nih.gov/articles/PMC49952/ | |
| dc.identifier | https://pubmed.ncbi.nlm.nih.gov/1382292/ | |
| dc.identifier | https://doi.org/10.1073/pnas.89.18.8522 | |
| dc.identifier.uri | https://repo.dare.co.zw/handle/123456789/158292 | |
| dc.language | en | |
| dc.publisher | National Academy of Sciences | |
| dc.source | Proc Natl Acad Sci U S A | |
| dc.subject | Research Article | |
| dc.title | Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies. | |
| dc.type | Text |