Cyclin D3 restricts SARS-CoV-2 envelope incorporation into virions and interferes with viral spread.

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dc.creatorGupta, Ravi K
dc.creatorMlcochova, Petra
dc.date2022-10-10T22:00:13Z
dc.date2022-10-10T22:00:13Z
dc.date2022-11-17
dc.date2022-05-12
dc.date2022-10-10T22:00:12Z
dc.date.accessioned2026-08-03T03:12:56Z
dc.descriptionFunder: Lister Institute of Preventive Medicine (Lister Institute); Id: http://dx.doi.org/10.13039/501100001255
dc.descriptionFunder: Rosetrees Trust (Rosetrees); Id: http://dx.doi.org/10.13039/501100000833
dc.descriptionThe COVID-19 pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a great threat to human health. The interplay between the virus and host plays a crucial role in successful virus replication and transmission. Understanding host-virus interactions are essential for the development of new COVID-19 treatment strategies. Here, we show that SARS-CoV-2 infection triggers redistribution of cyclin D1 and cyclin D3 from the nucleus to the cytoplasm, followed by proteasomal degradation. No changes to other cyclins or cyclin-dependent kinases were observed. Further, cyclin D depletion was independent of SARS-CoV-2-mediated cell cycle arrest in the early S phase or S/G2/M phase. Cyclin D3 knockdown by small-interfering RNA specifically enhanced progeny virus titres in supernatants. Finally, cyclin D3 co-immunoprecipitated with SARS-CoV-2 envelope (E) and membrane (M) proteins. We propose that cyclin D3 impairs the efficient incorporation of envelope protein into virions during assembly and is depleted during SARS-CoV-2 infection to restore efficient assembly and release of newly produced virions.
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dc.formatapplication/pdf
dc.identifierThe EMBO Journal, article-number e111653
dc.identifier0261-4189
dc.identifierembj2022111653
dc.identifierhttps://www.repository.cam.ac.uk/handle/1810/341882
dc.identifier10.17863/CAM.89306
dc.identifier1460-2075
dc.identifier.urihttps://repo.dare.co.zw/handle/123456789/174114
dc.languageen
dc.languageeng
dc.publisherSpringer Nature
dc.publisherhttps://doi.org/10.15252/embj.2022111653
dc.rightsAttribution 4.0 International
dc.rightshttps://creativecommons.org/licenses/by/4.0/
dc.subjectFucci
dc.subjectSARS-CoV-2
dc.subjectassembly
dc.subjectcell cycle
dc.subjectcyclin D3
dc.subjectHumans
dc.subjectSARS-CoV-2
dc.subjectCyclin D3
dc.subjectCOVID-19
dc.subjectPandemics
dc.subjectCell Line
dc.subjectVirion
dc.subjectCOVID-19 Drug Treatment
dc.titleCyclin D3 restricts SARS-CoV-2 envelope incorporation into virions and interferes with viral spread.
dc.typeArticle

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