Control of interferon-τ gene expression by Ets-2
| dc.creator | Ezashi, Toshihiko | |
| dc.creator | Ealy, Alan D. | |
| dc.creator | Ostrowski, Michael C. | |
| dc.creator | Roberts, R. Michael | |
| dc.date | 1998-07-07 | |
| dc.date.accessioned | 2026-08-02T20:19:57Z | |
| dc.description | Expression of the multiple interferon-τ (IFN-τ) genes is restricted to embryonic trophectoderm of ruminant ungulate species for a few days in early pregnancy. The promoter regions of these genes are highly conserved. A proximal (bp −91 to −69) sequence has been implicated in controlling trophoblast-specific expression. Here it was used as a target for yeast one-hybrid screening of a day 13 conceptus cDNA library. Two transcription factors of the Ets family, Ets-2 and GABPα, were identified, consistent with the observation that active ovine IFN-τ genes contain a single 10-bp Ets motif (core: GGAA) in the proximal segment, whereas three known inactive ovine genes contain a mutated core motif (TGAA). Cotransfection of a promoter- (−126 to +50) luciferase reporter construct from an active gene (bovineIFN-τ1; boIFNT1) and an Ets-2 expression plasmid in human JAr cells provided up to a 30-fold increase in reporter expression, whereas promoters from inactive genes were not transactivated. GABPα alone was ineffective and had only a ≈2-fold positive effect when coexpressed with its partner GABPβ. Other Ets-related transcription factors, which were not detected in the genetic screen, also provided a range of lesser transactivation effects. Coexpression of Ets-2 and activated Ras failed to transactivate the IFNT promoter greater than Ets-2 alone in JAr cells. The presence of Ets-2 in nuclei of embryonic trophectoderm was confirmed immunocytochemically. Together, these data suggest that Ets-2 plays a role in the transient expression of the nonvirally inducible IFNT genes. | |
| dc.identifier | https://pmc.ncbi.nlm.nih.gov/articles/PMC20898/ | |
| dc.identifier | https://pubmed.ncbi.nlm.nih.gov/9653109/ | |
| dc.identifier | https://doi.org/10.1073/pnas.95.14.7882 | |
| dc.identifier.uri | https://repo.dare.co.zw/handle/123456789/85609 | |
| dc.language | en | |
| dc.publisher | National Academy of Sciences | |
| dc.rights | Copyright © 1998, The National Academy of Sciences | |
| dc.source | Proc Natl Acad Sci U S A | |
| dc.subject | Biological Sciences | |
| dc.title | Control of interferon-τ gene expression by Ets-2 | |
| dc.type | Text |