Tolerance induction by thymic medullary epithelium.
| dc.creator | Hoffmann, M W | |
| dc.creator | Allison, J | |
| dc.creator | Miller, J F | |
| dc.date | 1992-04-01 | |
| dc.date.accessioned | 2026-08-03T01:30:11Z | |
| dc.description | To study the role of thymic medullary epithelium in tolerance induction, the third and fourth branchial clefts of embryos from E mu-Kb transgenic mice, which express the major histocompatibility complex class I antigen H-2Kb exclusively on medullary thymic epithelium, were grafted to athymic nude mice. The grafts differentiated into tissue that morphologically resembled normal thymus. These grafts expressed the H-2Kb antigen appropriately and gave rise to a functional T cell repertoire. In vivo tolerance to H-2Kb disparate skin grafts was invariably found in mice expressing H-2Kb in the medulla or in both medulla and cortex of C57BL/6 branchial cleft-grafted controls. In marked contrast, in vitro cytotoxicity assays demonstrated reactivity toward H-2Kb in the presence of interleukin 2, and limiting-dilution analyses showed similar frequencies of cytolytic T cell precursors reactive to H-2Kb and to third-party stimulators. Medullary epithelium can, therefore, induce split tolerance, in which in vivo tolerance is accompanied by strong in vitro responses in the presence of interleukin 2. IMAGES: | |
| dc.identifier | https://pmc.ncbi.nlm.nih.gov/articles/PMC48694/ | |
| dc.identifier | https://pubmed.ncbi.nlm.nih.gov/1557355/ | |
| dc.identifier | https://doi.org/10.1073/pnas.89.7.2526 | |
| dc.identifier.uri | https://repo.dare.co.zw/handle/123456789/154915 | |
| dc.language | en | |
| dc.publisher | National Academy of Sciences | |
| dc.source | Proc Natl Acad Sci U S A | |
| dc.subject | Research Article | |
| dc.title | Tolerance induction by thymic medullary epithelium. | |
| dc.type | Text |