CD40 ligand (CD154) stimulation of macrophages to produce HIV-1-suppressive β-chemokines
| dc.creator | Kornbluth, Richard S. | |
| dc.creator | Kee, Kristin | |
| dc.creator | Richman, Douglas D. | |
| dc.date | 1998-04-28 | |
| dc.date.accessioned | 2026-08-02T20:12:23Z | |
| dc.description | β-chemokines play an important role in the development of immunologic reactions. Macrophages are major β-chemokine-producing cells during T-cell directed, delayed-type hypersensitivity reactions in tissues, and have been reported to be important producers of β-chemokines in the lymph nodes of HIV-1-infected individuals. However, the physiological signals responsible for inducing macrophages to produce β-chemokines have not been established. Two soluble T cell products, interferon-γ and granulocyte-macrophage colony stimulating factor, were added to cultured macrophages, but failed to stimulate the production of macrophage inflammatory protein-1α and -1β; regulated upon activation, normal T cell expressed and secreted (RANTES); or monocyte chemoattractant protein-1. Instead, direct cell–cell contact between macrophages and cells engineered to express CD40L (also known as CD154) resulted in the production of large amounts of macrophage inflammatory protein-1α and -1β, and RANTES (all ligands for CCR5), and monocyte chemoattractant protein-1 (a ligand for CCR2). Supernatants from CD40L-stimulated macrophages protected CD4(+) T cells from infection by a nonsyncytium-inducing strain of HIV-1 (which uses CCR5 as a coreceptor). These results have implications for granulomatous diseases, and conditions such as atherosclerosis and multiple sclerosis, where CD40L-bearing cells have been found in the macrophage-rich lesions where β-chemokines are being produced. Overall, these findings define a pathway linking the specific recognition of antigen by T cells to the production of β-chemokines by macrophages. This pathway may play a role in anti-HIV-1 immunity and the development of immunologic reactions or lesions. | |
| dc.identifier | https://pmc.ncbi.nlm.nih.gov/articles/PMC20239/ | |
| dc.identifier | https://pubmed.ncbi.nlm.nih.gov/9560254/ | |
| dc.identifier | https://doi.org/10.1073/pnas.95.9.5205 | |
| dc.identifier.uri | https://repo.dare.co.zw/handle/123456789/83842 | |
| dc.language | en | |
| dc.publisher | National Academy of Sciences | |
| dc.rights | Copyright © 1998, The National Academy of Sciences | |
| dc.source | Proc Natl Acad Sci U S A | |
| dc.subject | Biological Sciences | |
| dc.title | CD40 ligand (CD154) stimulation of macrophages to produce HIV-1-suppressive β-chemokines | |
| dc.type | Text |